If all forward and reverse ABO results as well as the autocontrol are positive, what should be done?

If all forward and reverse ABO results as well as the autocontrol are positive, what should be done?

• A. Wash the cells with warm saline and autoadsorb serum
• B. Retype the sample using different reagents
• C. Use polyclonal typing reagents
• D. Report as group AB

Answer: (A)

In situations where all forward and reverse ABO results, along with the autocontrol, return positive, additional steps must be taken to clarify the blood group. Washing the cells with warm saline and performing an autoadsorption is a logical approach in this scenario. When both forward and reverse typing yield positive results, it raises the possibility of the presence of autoantibodies or a nonspecific reaction. By washing the cells, you can help remove any nonspecific antibodies that might be causing false-positive reactions. The subsequent step of autoadsorbing the serum allows for the removal of these autoantibodies, as the patient's red blood cells will “adsorb” their antibodies onto their own cells, enabling more accurate ABO typing. This approach is essential to ensure that the ABO typing reflects the true blood group of the patient, rather than being skewed by unusual serological reactions.

In situations where all forward and reverse ABO results, along with the autocontrol, return positive, additional steps must be taken to clarify the blood group. Washing the cells with warm saline and performing an autoadsorption is a logical approach in this scenario.

When both forward and reverse typing yield positive results, it raises the possibility of the presence of autoantibodies or a nonspecific reaction. By washing the cells, you can help remove any nonspecific antibodies that might be causing false-positive reactions. The subsequent step of autoadsorbing the serum allows for the removal of these autoantibodies, as the patient's red blood cells will “adsorb” their antibodies onto their own cells, enabling more accurate ABO typing.

This approach is essential to ensure that the ABO typing reflects the true blood group of the patient, rather than being skewed by unusual serological reactions.