In a scenario where screening cells and major crossmatch are positive on IS only, and the autocontrol is negative, what should be identified?

In a scenario where screening cells and major crossmatch are positive on IS only, and the autocontrol is negative, what should be identified?

• A. Cold alloantibody
• B. Cold autoantibody
• C. Abnormal protein
• D. Antibody mixture

Answer: (A)

In this scenario, where screening cells and major crossmatch are positive on immediate spin (IS) only and the autocontrol is negative, the identification of a cold alloantibody is appropriate. When testing scenarios present with positive reactions at immediate spin, it often indicates that the antibodies involved are reacting with red blood cell antigens under cold temperatures. The negative autocontrol suggests that the patient's own red blood cells are not showing reactivity with the antibodies, which points away from an autoantibody scenario. Cold alloantibodies are often IgM antibodies that can lead to positive reactions with certain antigens present on screening cells or donor red blood cells during the IS phase. These reactions typically diminish or disappear upon warming to body temperature or in further testing at 37°C, which is consistent with the findings of a screening and crossmatch positivity in cold conditions only. In contrast, cold autoantibodies would show reactivity in the autocontrol, as they are derived from the patient's own immune response, reacting with their own red blood cell antigens. Abnormal proteins might have different implications that do not specifically relate to the immediate spin phase or the autocontrol results. An antibody mixture would suggest more complex patterns of reactivity than what is indicated

In this scenario, where screening cells and major crossmatch are positive on immediate spin (IS) only and the autocontrol is negative, the identification of a cold alloantibody is appropriate.

When testing scenarios present with positive reactions at immediate spin, it often indicates that the antibodies involved are reacting with red blood cell antigens under cold temperatures. The negative autocontrol suggests that the patient's own red blood cells are not showing reactivity with the antibodies, which points away from an autoantibody scenario.

Cold alloantibodies are often IgM antibodies that can lead to positive reactions with certain antigens present on screening cells or donor red blood cells during the IS phase. These reactions typically diminish or disappear upon warming to body temperature or in further testing at 37°C, which is consistent with the findings of a screening and crossmatch positivity in cold conditions only.

In contrast, cold autoantibodies would show reactivity in the autocontrol, as they are derived from the patient's own immune response, reacting with their own red blood cell antigens. Abnormal proteins might have different implications that do not specifically relate to the immediate spin phase or the autocontrol results. An antibody mixture would suggest more complex patterns of reactivity than what is indicated