To confirm the presence of anti-Jka antibodies, which method would be least effective?

To confirm the presence of anti-Jka antibodies, which method would be least effective?

• A. Testing with enzyme-treated cells
• B. Select panel of homozygous cells
• C. Testing with AET-treated cells
• D. Increased incubation time

Answer: (C)

The confirmation of anti-Jka antibodies involves utilizing methods that enhance the reaction specificity and sensitivity. Enzyme-treated cells can modify the red blood cell surface and enhance the antigen-antibody reaction, making them effective in confirming the presence of specific antibodies. Utilizing a select panel of homozygous cells is also advantageous since these cells express the Jka antigen in a more robust manner, allowing for clearer identification of the antibody presence through more pronounced reactions. Increased incubation time can improve the interaction between antibodies and their corresponding antigens, thereby enhancing the likelihood of detecting the antibody if it is indeed present. In contrast, testing with AET (2-aminoethylisothiouronium bromide) - treated cells is least effective in this scenario. AET is a reducing agent that disrupts disulfide bonds on the red blood cell membrane, which can lead to a loss of reactivity of certain blood group antigens. This destruction or alteration of antigenic sites can hinder the ability to confirm the presence of anti-Jka antibodies, ultimately making this method inadequate for confirming the antibodies when compared to the other options.

The confirmation of anti-Jka antibodies involves utilizing methods that enhance the reaction specificity and sensitivity. Enzyme-treated cells can modify the red blood cell surface and enhance the antigen-antibody reaction, making them effective in confirming the presence of specific antibodies.

Utilizing a select panel of homozygous cells is also advantageous since these cells express the Jka antigen in a more robust manner, allowing for clearer identification of the antibody presence through more pronounced reactions.

Increased incubation time can improve the interaction between antibodies and their corresponding antigens, thereby enhancing the likelihood of detecting the antibody if it is indeed present.

In contrast, testing with AET (2-aminoethylisothiouronium bromide) - treated cells is least effective in this scenario. AET is a reducing agent that disrupts disulfide bonds on the red blood cell membrane, which can lead to a loss of reactivity of certain blood group antigens. This destruction or alteration of antigenic sites can hinder the ability to confirm the presence of anti-Jka antibodies, ultimately making this method inadequate for confirming the antibodies when compared to the other options.