What procedure would be most suitable for distinguishing anti-I antibodies from other similar antibodies in a recent transfusion patient?

What procedure would be most suitable for distinguishing anti-I antibodies from other similar antibodies in a recent transfusion patient?

• A. Autoadsorption technique
• B. Neutralization using saliva
• C. Autocontrol using ZZAP reagent-treated cells
• D. Reaction with cord cells

Answer: (D)

The most suitable procedure for distinguishing anti-I antibodies from other similar antibodies in a recent transfusion patient is the reaction with cord cells. Anti-I antibodies are known to react with adult red blood cells but not with cord blood cells. This is due to the expression of I antigen, which is present in adult red blood cells but largely absent in cord blood cells. When testing serum from a recent transfusion patient, if the serum reacts with adult red cells but not with cord blood cells, it indicates the presence of an anti-I antibody. This clear distinction helps in confirming the presence of the specific antibody in question. The other methods, while useful in different contexts, do not specifically target the ability of anti-I antibodies to differentiate themselves from other types of antibodies as effectively as the reaction with cord cells does. For instance, autoadsorption might not adequately separate different antibodies if they are present in the patient's serum. Neutralization using saliva can help identify certain antibodies but may not be effective for anti-I. Using ZZAP reagent-treated cells generally helps to identify warm autoantibodies but may not provide precise differentiation for antibodies like anti-I. Hence, reaction with cord cells remains the most reliable method in this scenario.

The most suitable procedure for distinguishing anti-I antibodies from other similar antibodies in a recent transfusion patient is the reaction with cord cells. Anti-I antibodies are known to react with adult red blood cells but not with cord blood cells. This is due to the expression of I antigen, which is present in adult red blood cells but largely absent in cord blood cells. When testing serum from a recent transfusion patient, if the serum reacts with adult red cells but not with cord blood cells, it indicates the presence of an anti-I antibody. This clear distinction helps in confirming the presence of the specific antibody in question.

The other methods, while useful in different contexts, do not specifically target the ability of anti-I antibodies to differentiate themselves from other types of antibodies as effectively as the reaction with cord cells does. For instance, autoadsorption might not adequately separate different antibodies if they are present in the patient's serum. Neutralization using saliva can help identify certain antibodies but may not be effective for anti-I. Using ZZAP reagent-treated cells generally helps to identify warm autoantibodies but may not provide precise differentiation for antibodies like anti-I. Hence, reaction with cord cells remains the most reliable method in this scenario.