Which enhancement medium decreases the zeta potential to facilitate interaction between antibody and antigen?

Which enhancement medium decreases the zeta potential to facilitate interaction between antibody and antigen?

• A. LISS
• B. Polyethylene glycol
• C. Polybrene
• D. ZZAP

Answer: (A)

LISS, or Low Ionic Strength Solution, is specifically designed to decrease the zeta potential between red blood cells and antibodies. The zeta potential is a measure of the electrostatic repulsion or attraction between charged particles in solution. By providing a low ionic strength environment, LISS reduces the charge repulsion between the red blood cells and antibodies. This allows for closer proximity and increases the likelihood of antigen-antibody interactions, thereby enhancing agglutination reactions in serological tests. In contrast, polyethylene glycol, while it can enhance agglutination, works through a different mechanism by promoting the aggregation of cells rather than specifically addressing the zeta potential. Polybrene also serves to increase the rate of agglutination but primarily through the neutralization of charge rather than altering the ionic strength of the medium. ZZAP is used for different purposes, including the removal of specific antibodies but does not serve as an enhancement medium in the same way that LISS does. Overall, LISS is uniquely effective in its role of decreasing zeta potential, which is crucial for facilitating antigen-antibody interactions.

LISS, or Low Ionic Strength Solution, is specifically designed to decrease the zeta potential between red blood cells and antibodies. The zeta potential is a measure of the electrostatic repulsion or attraction between charged particles in solution. By providing a low ionic strength environment, LISS reduces the charge repulsion between the red blood cells and antibodies. This allows for closer proximity and increases the likelihood of antigen-antibody interactions, thereby enhancing agglutination reactions in serological tests.

In contrast, polyethylene glycol, while it can enhance agglutination, works through a different mechanism by promoting the aggregation of cells rather than specifically addressing the zeta potential. Polybrene also serves to increase the rate of agglutination but primarily through the neutralization of charge rather than altering the ionic strength of the medium. ZZAP is used for different purposes, including the removal of specific antibodies but does not serve as an enhancement medium in the same way that LISS does.

Overall, LISS is uniquely effective in its role of decreasing zeta potential, which is crucial for facilitating antigen-antibody interactions.