Which method is used to confirm the specificity of weakly reactive antibodies in serum?

The correct method for confirming the specificity of weakly reactive antibodies in serum is cold adsorption. In this technique, serum that contains weakly reactive antibodies is mixed with red blood cells that are known to express the corresponding antigens. By incubating at lower temperatures (usually around 4 degrees Celsius), only the weakly binding antibodies remain associated with the cells, while other antibodies can be removed. This helps to isolate the weakly reactive antibodies and determine their specificity.

Cold adsorption is particularly useful in scenarios where the weak antibody reaction is not easily distinguished due to the presence of other antibodies or factors within the serum. This method allows for a clearer interpretation of the immune response by specifically focusing on the antibodies of interest.

Weak D testing, antibody elution, and direct antigen typing are all important techniques in immunohematology, but they serve different purposes. Weak D testing is typically used to determine the Rh status of a blood sample, particularly when the D antigen is weakly expressed. Antibody elution is primarily used to recover antibodies from red blood cells in cases where they are no longer detectable in serum, usually to identify antibodies that were bound in vivo. Direct antigen typing is used to determine the presence of specific antigens on red blood cells. While