Which of the following is NOT a reason for performing an adsorption?

Which of the following is NOT a reason for performing an adsorption?

• A. Separation of mixtures of antibodies
• B. Removal of interfering substances
• C. Confirmation of weak antigens on red cells
• D. Identification of antibodies causing a positive DAT

Answer: (D)

Performing an adsorption technique primarily serves specific ends in the context of immunology and transfusion medicine. The correct answer—identifying antibodies causing a positive direct antiglobulin test (DAT)—is not a purpose of adsorption. While adsorption is useful for removing interfering antibodies and clarifying the specificity of antibody populations, its primary role does not pertain to identifying the specific antibodies involved in a positive DAT. Adsorption is frequently used to achieve separation of antibody mixtures or to eliminate non-specific antibodies that may confound test results. Additionally, it aids in confirming weak antigens on red blood cells by enhancing the visibility of limited antigen-antibody interactions. In contrast, the positive DAT indicates that antibodies (or complement) are bound to red cells in vivo, and further investigation would typically involve performing additional testing methods, rather than employing adsorption to clarify or identify these antibodies.

Performing an adsorption technique primarily serves specific ends in the context of immunology and transfusion medicine. The correct answer—identifying antibodies causing a positive direct antiglobulin test (DAT)—is not a purpose of adsorption. While adsorption is useful for removing interfering antibodies and clarifying the specificity of antibody populations, its primary role does not pertain to identifying the specific antibodies involved in a positive DAT.

Adsorption is frequently used to achieve separation of antibody mixtures or to eliminate non-specific antibodies that may confound test results. Additionally, it aids in confirming weak antigens on red blood cells by enhancing the visibility of limited antigen-antibody interactions. In contrast, the positive DAT indicates that antibodies (or complement) are bound to red cells in vivo, and further investigation would typically involve performing additional testing methods, rather than employing adsorption to clarify or identify these antibodies.