Which procedure would help to distinguish between an anti-e and anti-Fya in an antibody mixture?

To distinguish between anti-e and anti-Fya in an antibody mixture, running an enzyme panel is an effective procedure. This is because both antibodies demonstrate different reactivity patterns when exposed to specific enzymes that alter the structure of red blood cell antigens.

Enzyme panels typically involve using enzymes like ficin or papain, which enhance the expression of certain antigens while potentially diminishing the effect of other antibodies. In the case of anti-Fya, enzyme treatment can enhance the expression of Fya antigen, making it easier to detect its presence or absence. Conversely, anti-e may show altered reactivity due to the specific nature of the antigens on the red blood cells after enzyme treatment.

Thus, by observing the reactions of the panel, one can differentiate between anti-e and anti-Fya based on how they respond to the alterations caused by the enzymatic treatment. This allows for a clearer identification of the antibodies present in the mixture.

Lowering the pH of the test serum may alter the environment, but it is not a reliable method for distinguishing between these two specific antibodies. Using a thiol reagent primarily disrupts disulfide bonds and may not provide the distinct reactions needed to differentiate between anti-e and anti-Fya. Running a LISS (